Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.
نویسندگان
چکیده
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.
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عنوان ژورنال:
- Journal of the American Chemical Society
دوره 133 27 شماره
صفحات -
تاریخ انتشار 2011